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A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
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JoVE Journal Biologie
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

DOI:
10.3791/52161-v

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07:24 min

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December 10, 2014

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, , , , ,
1Egg Safety and Quality Research Unit,USDA-Agricultural Research Service, 2Poultry Microbiological Safety and Processing Research Unit,USDA-Agricultural Research Service, 3Department of Biochemistry and Biophysics,Oregon State University, 4College of Public Health,University of Georgia, 5Department of Biological Sciences, Center for Microbial Genetics and Genomics,Northern Arizona University

Kapitel

  • 00:05Titel
  • 01:56Mechanical Homogenization
  • 03:32Enzymatic Inhibition of Inhibitors from Sample Homogenates
  • 04:51Automated DNA Purification
  • 06:25Results: Hybrid DNA Extraction Method Effective for Total Bacterial Assessment
  • 06:54Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A novel semi-automated hybrid DNA extraction method for use with environmental poultry production samples was developed and demonstrated improvements over a common mechanical and enzymatic extraction method in terms of the quantitative and qualitative estimates of the total bacterial communities.

Tags

Keywords: DNA ExtractionBacterial CommunitiesPoultry ProductionHybrid MethodMechanical HomogenizationEnzymatic ExtractionAutomated Purification16S RRNA QPCRMicrobiomics

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