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Kullanımı Geleneksel Hikaye ve Roman Yerleşimi Sistemleri: Plak Tahliller sayesinde viral Konsantrasyon Tayini
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems

Kullanımı Geleneksel Hikaye ve Roman Yerleşimi Sistemleri: Plak Tahliller sayesinde viral Konsantrasyon Tayini

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09:28 min

November 04, 2014

DOI:

09:28 min
November 04, 2014

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Summary

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Plaque assays are the gold standard for viral quantification, utilizing entrapping overlays on host cellular monolayers to determine viral titers. While various semisolid overlays have traditionally been used, here we demonstrate plaque techniques comparing semisolid overlays to a novel liquid microcrystalline cellulose among several families of viruses.

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