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Analysen av Neurokar ombygging i Entorhino-hippocampus organotypic skive kulturer
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
DOI:

08:27 min

October 23, 2014

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Kapitel

  • 00:05Titel
  • 01:20Organotypic Entorhino-hippocampal Slice Cultures
  • 02:37In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
  • 04:28Fixation and Immunostaining
  • 05:47Determination of Regional Vessel Density
  • 06:22Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
  • 07:41Conclusion

Summary

Automatische Übersetzung

En protokoll for entorhino-hippocampus organotypic skive kulturer, som lar reprodusere mange aspekter av iskemisk hjerneskade, blir presentert. Ved å studere forandringer i neurovasculature i tillegg til endringer i neuroner, er denne protokollen et allsidig verktøy for å studere forandringer i plast neural vev etter skade.

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