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Emparelhados Recordings células inteiras em organot�icas fatias do hipocampo
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Kapitel
- 00:05Titel
- 01:16Making Hippocampal Slices
- 03:50Handling Hippocampal Slices
- 05:11Whole Cell Recordings in Hippocampal Slices
- 07:39Synaptic Connectivity Between CA3 Pyramidal Neurons
- 09:00Conclusion
Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.
