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Høy gjennomstrømming analysen til sin fenotype<em> Salmonella ente</em> Typhimurium Association, Invasion, og Replication i makrofager
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Høy gjennomstrømming analysen til sin fenotype<em> Salmonella ente</em> Typhimurium Association, Invasion, og Replication i makrofager
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Microbial and Molecular Pathogenesis, College of Medicine,Texas A&M University System Health Science Center, 3University of California, Irvine, 4Department of Medical Microbiology & Immunology, School of Medicine,University of California, Davis
Kapitel
- 00:05Titel
- 01:59Murine Macrophage RAW264.7 Cell Culture
- 03:29Preparation of Salmonella Wild-type and Mutants
- 05:19Invasion Assay in a 96-well Culture Plate
- 09:58Results: Identification of the Phenotype of Salmonella Mutants
- 10:46Conclusion
En high-throughput analysen til in vitro fenotype Salmonella eller annen bakteriell forening, invasjon, og replikering i fagocyttiske celler med høy gjennomstrømming kapasitet ble utviklet. Metoden ble ansatt for å evaluere Salmonella genet knockout mutante stammer for sine engasjementer i verts-patogen interaksjoner.
