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Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

68,618 Views

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09:32 min

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September 09, 2014

DOI:

10.3791/51554-v

DOI:
10.3791/51554-v

•

09:32 min
September 09, 2014

•

7 Views
, , , , , ,
1Department of Hematology and Oncology,University Medical Center Göttingen, 2Microbial Interface Biology Group,Research Center Borstel

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Human MonocytesDouble Gradient CentrifugationMacrophage DifferentiationTeflon-coated Cell Culture BagsM-CSFMacrophage IsolationQuality ControlSyngeneic Human System

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Menck, K., Behme, D., Pantke, M., Reiling, N., Binder, C., Pukrop, T., Klemm, F. Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags. J. Vis. Exp. (91), e51554, doi:10.3791/51554 (2014).

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