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رصد Juxtacellular وتوطين واحدة الخلايا العصبية داخل الدماغ الهياكل الفرعية القشرية للتنبيه، الجرذان-ضبط النفس رئيس
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JoVE Journal Neurowissenschaften
Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats
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رصد Juxtacellular وتوطين واحدة الخلايا العصبية داخل الدماغ الهياكل الفرعية القشرية للتنبيه، الجرذان-ضبط النفس رئيس

11,177 Views

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08:41 min

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April 27, 2015

DOI:

10.3791/51453-v

DOI:
10.3791/51453-v

•

08:41 min
April 27, 2015

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3 Views
, ,
1Department of Physics 0374,University of California, San Diego, 2Department of Psychiatry and Neuroscience,Centre de Recherche de l’Université Laval Robert-Giffard

Summary

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This protocol describes the design and surgical implantation of a head-restraining mechanism to monitor neuronal activity in sub-cortical brain structures in alert rats. It delineates procedures to isolate single neurons in the juxtacellular configuration and to efficiently identify their anatomical locations.

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Moore, J. D., Deschênes, M., Kleinfeld, D. Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats. J. Vis. Exp. (98), e51453, doi:10.3791/51453 (2015).

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