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囊性纤维化跨膜电导调节蛋白表达纯化<em>酿酒酵母</em
JoVE Journal
Biologie
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JoVE Journal Biologie
Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae
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囊性纤维化跨膜电导调节蛋白表达纯化<em>酿酒酵母</em

DOI:
10.3791/51447-v

•

15:12 min

•

May 10, 2014

•

, , ,
1Faculty of Life Sciences,University of Manchester

Kapitel

  • 00:05Titel
  • 01:46Solubilization of Microsomes
  • 03:49Nickel-affinity Purification of CFTR
  • 05:33Gel Permeation Chromatography (GPC) Purification of CFTR
  • 06:57Reconstitution of CFTR
  • 07:51Measurement of ATPase Activity
  • 09:35Results: Isolation of Milligram Quantities of CFTR by Two Methods
  • 14:28Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

异源表达和囊性纤维化跨膜电导调节器（CFTR）的净化是显著的挑战和制约因素的药物治疗囊性纤维化的发展。这个协议描述了两种方法的CFTR的毫克量适合于功能和结构研究的分离。

Tags

Cystic FibrosisCFTR ProteinMembrane Protein PurificationSaccharomyces CerevisiaeDetergent SolubilizationDDMLPG-14ATPase ActivityPost-translational ModificationsStructural Analysis

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