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En Flow Vedhæftning Assay til at studere Leucocyte Rekruttering til human hepatiske Sinusformede endotel Under betingelser for forskydningsspænding
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Kapitel
- 00:05Titel
- 01:59Cytokine Stimulation of Endothelial Cells
- 04:11Isolation and Pretreatment of Peripheral Blood Lymphocytes
- 06:09Setup of Flow Assay System
- 08:33Flow AssayTechnique and Recording of Adhesion for Analysis
- 09:35Results: Adhesion Cascade of Blood Lymphocytes over Hepatic Sinusoidal Endothelium
- 10:44Conclusion
Leucocyte rekruttering til leveren sker inden de specialiserede kanaler leverens sinusoider, som er foret med unikke hepatiske sinusformede endotelceller. Fasekontrastmikroskopi af leukocyt rekruttering på tværs af human hepatisk sinusformet endotel under betingelser med fysiologisk forskydningsspænding kan lette opklaringen af de molekylære mekanismer, der ligger til grund for denne proces.
Tags
Flow Adhesion AssayLeucocyte RecruitmentHuman Hepatic Sinusoidal EndotheliumShear StressInflammatory Liver DiseasesFibrosisCirrhosisMolecular MechanismsTherapeutic TargetsLeucocyte InfiltrationHepatic SinusoidsHepatic Sinusoidal Endothelial Cells (HSEC)Adhesion CascadeTetheringActivationFirm AdhesionCrawlingTransmigrationPhase Contrast MicroscopyInhibitors
