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שימוש בcaspase ריבוב Assay לקביעת אפופטוזיס בדגם נייד ההיפותלמוס
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Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
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שימוש בcaspase ריבוב Assay לקביעת אפופטוזיס בדגם נייד ההיפותלמוס

14,858 Views

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08:27 min

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April 16, 2014

DOI:

10.3791/51305-v

DOI:
10.3791/51305-v

•

08:27 min
April 16, 2014

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4 Views
, , , , ,
1Department of Veterans Affairs,Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition,University of Minnesota, 3Department of Integrative Biology and Physiology,University of Minnesota, 4Department of Medicine, University of Minnesota Medical School,University of Minnesota

Tags

Caspase MultiplexingApoptosisHypothalamic Cell ModelPalmitic AcidResazurinCaspase-3/7Luminogenic SubstrateDEVDSaturated Fatty AcidOxidative Stress

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Butterick, T. A., Duffy, C. M., Lee, R. E., Billington, C. J., Kotz, C. M., Nixon, J. P. Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model. J. Vis. Exp. (86), e51305, doi:10.3791/51305 (2014).

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