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An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans
 

An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans

Article DOI: 10.3791/51231-v • 09:24 min • March 11th, 2014
March 11th, 2014 •
1, 1, 2
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 2Department of Medicine, Nephrology Division, University of Rochester Medical Center, School of Medicine and Dentistry

Kapitel

Summary
Automatic Translation

A protocol is described that uses anoxia/starvation in C. elegans to model ischemia/reperfusion. Functional outcomes include increased mortality, visible abnormalities in GFP-labeled neuronal processes, and impaired behavioral responses that require neuronal function.

Tags

Anoxia/starvation Model Ischemia/reperfusion C. Elegans Genetic Model Organism Worm Bacterial Food Anoxia Simulated Ischemia Normal Atmosphere Simulated Reperfusion Death Neuronal Damage Organism Viability Touch Neuron Processes Touch Sensitivity Behavioral Output Hypoxic Incubators Kitchen Storage Containers Mass Flow Control Unit Gas Mixture Custom Incubators Circulating Water Bath Temperature Control Leaks
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