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Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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JoVE Journal Neurowissenschaften
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

DOI:
10.3791/51091-v

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13:22 min

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January 23, 2014

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, , ,
1Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire,CNRS UMR 5534, 2Université de Lyon 1, 3Laboratoire d’excellence,LabEX DEVweCAN, 4Institut de Virologie Moléculaire et Structurale,CNRS UPR 3296, 5Centre de Recherche en Cancérologie de Lyon, INSERM U1052,CNRS UMR 5286

Kapitel

  • 00:05Titel
  • 02:27Animal Infection
  • 03:20Trigeminal Ganglia Harvesting and Sectioning
  • 05:02DNA-FISH
  • 10:08Protocol Development and Applications
  • 12:32Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.

Tags

Keywords: DNA-FISHRNA-FISHImmunofluorescenceHSV-1LatencyGene Expression RegulationSingle Cell Analysis

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