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Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
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Biologie
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JoVE Journal Biologie
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

DOI:
10.3791/50971-v

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07:33 min

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January 03, 2014

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, ,
1Wageningen UR Plant Breeding,Wageningen University, 2Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, National Centre for Vegetable Improvement (Central China), Potato Engineering and Technology Research Centre of Hubei Province,Huazhong Agricultural University

Kapitel

  • 00:05Titel
  • 01:25Plant Growing, Testing Conditions, and Agroinfiltration
  • 04:11PVX Agroinfection
  • 05:27Representative Agroinfiltration Results
  • 06:54Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Agroinfiltration and PVX agroinfection are routine functional assays for transient ectopic expression of genes in plants. These methods are efficient assays in effectoromics strategies (rapid resistance and avirulence gene discovery) and crucial to modern research in molecular plant pathology. They meet the demand for robust high-throughput functional analysis in plants.

Tags

Keywords: AgroinfiltrationPVX AgroinfectionPotatoNicotiana BenthamianaTransient ExpressionFunctional AnalysisAgrobacterium TumefaciensR-Avr InteractionsNonspecific Defense ResponsesVirus-induced Extreme Resistance

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