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Synthesis, Cellular Levering og<em> In vivo</em> Anvendelse af Dendrimer-baserede pH-sensorer
1Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry,Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 2NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST,Center for Nanotechnology Innovation, 3NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, 4IIT@NEST,Center for Nanotechnology Innovation
Kapitel
- 00:05Titel
- 02:30Synthesis of the Sensors
- 04:20In vitro pH Measurements
- 05:36Cell Culture and Electroporation
- 06:38pH Sensing in Living HeLa Cells
- 08:47In vivo Sample Preparation
- 10:30pH Imaging in Mouse Brain
- 12:24Representative Analysis of Dendrimer-based pH Sensor Fluorescence
- 15:45Conclusion
Fluorescens sensorer er stærke værktøjer i life science. Her beskriver vi en metode til at syntetisere og anvende dendrimer-baserede fluorescerende sensorer til at måle pH i levende celler og in vivo. Dendritiske stillads forbedrer egenskaberne af konjugerede fluorescerende farvestoffer, der fører til forbedrede sensing egenskaber.
