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可视化和分析的mRNA分子,使用荧光<em>在原位</em>杂交<em>酿酒酵母(Saccharomyces cerevisiae)</em
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Visualization and Analysis of mRNA Molecules Using Fluorescence
In Situ
Hybridization in
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
可视化和分析的mRNA分子,使用荧光<em>在原位</em>杂交<em>酿酒酵母(Saccharomyces cerevisiae)</em
DOI:
10.3791/50382-v
•
07:00 min
•
June 14, 2013
•
R. Scott McIsaac
2
,
Sanford J. Silverman
,
Lance Parsons
,
Ping Xu
,
Ryan Briehof
,
Megan N. McClean
,
David Botstein
3
1
The Lewis-Sigler Institute for Integrative Genomics
,
Princeton University
,
2
Graduate Program in Quantitative and Computational Biology
,
Princeton University
,
3
Department of Molecular Biology
,
Princeton University
Kapitel
00:05
Titel
01:11
Coverslip Preparation
01:51
Fixation
03:08
Hybridization
04:53
Imaging of Cells with Olympus IX-81 Inverted Microscope
05:23
Results: Quantitative mRNA Analysis by FISH
06:31
Conclusion
Summary
Automatische Übersetzung
English (Original)
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Automatische Übersetzung
本协议描述的实验过程进行荧光<em在原位</em>杂交(FISH)技术在单细胞单分子分辨率计数的mRNA。
Tags
Fluorescence In Situ Hybridization
FISH
MRNA
Saccharomyces Cerevisiae
Single-cell Analysis
Single-molecule
Spot Detection
Gene Expression
Visualization
Quantification
Article
Embed
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剖析
酿酒酵母
ASCI
荧光
原位
杂交(FISH)的议定书“在人类精子
脂肪酸定量磷酸化刺激
酿酒酵母</em
微阵列分析FOR
酿酒酵母</em
在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母
荧光检测病毒RNA<em>原位</em>杂交(FISH)
耦合检测监控蛋白复性<em>酿酒酵母(Saccharomyces cerevisiae)</em
mRNA的衰变率的测量<em>酿酒酵母</em>使用<em> rpb1-1</em>株
对于荧光DNA的简易方法<em>原位</em>杂交来压扁染色体
化学遗传相互作用的快速鉴定<em>酿酒酵母</em
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