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Generering af Topisk Transgene rotter ved<em> In Utero</em> Elektroporation og<em> In vivo</em> Bioluminescens Screening
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Neurowissenschaften
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JoVE Journal Neurowissenschaften
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Generering af Topisk Transgene rotter ved<em> In Utero</em> Elektroporation og<em> In vivo</em> Bioluminescens Screening

DOI:
10.3791/50146-v

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08:44 min

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September 24, 2013

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, , , ,
1Department of Neuropathology,Medical School Düsseldorf, 2Department of Molecular Genetics,Weizmann Institute for Science, 3Center of Behavioral Neuroscience,University of Düsseldorf

Kapitel

  • 00:05Titel
  • 02:01In utero Electroporation (IUE)
  • 03:10Bioluminescence Live Imaging of the Enzymatic Luciferase Reaction
  • 05:47Analysis of Bioluminescence Images
  • 06:41Results: Luminescence Detection After Electroporation
  • 08:01Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Gener kan manipuleres under udviklingen af cortex eller hippocampus fra rotte via in utero elektroporation (IUE) ved E16, således at hurtige og målrettede ændringer i neuronal tilslutning til senere undersøgelser af adfærd eller neuropatologi hos voksne dyr. Postnatal in vivo-billeddannelse til styring af IUE succes udføres ved bioluminescens aktivere cotransficeret luciferase.

Tags

Keywords: In Utero ElectroporationBioluminescence ImagingTransgenic RatsDISC1NeurodevelopmentBehavioral Neuroscience

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