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基因捕获斑马鱼中使用的Gal4
JoVE Journal
Biologie
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JoVE Journal Biologie
Gene Trapping Using Gal4 in Zebrafish
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基因捕获斑马鱼中使用的Gal4

17,928 Views

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13:34 min

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September 29, 2013

DOI:

10.3791/50113-v

DOI:
10.3791/50113-v

•

13:34 min
September 29, 2013

•

7 Views
,
1Department of Biology,Temple University

Summary

Automatically generated

本协议描述的使用GAL4-VP16为主要记者和GFP / RFP在斑马鱼次要记者基因陷阱插入突变的方法。约于一体的高表达F0鱼产量的基因陷阱后代共同表达GFP和RFP十名。筛选过程可以容易地缩放，以适应执行插入诱变屏幕实验室的规模。

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Cite this Article

Balciuniene, J., Balciunas, D. Gene Trapping Using Gal4 in Zebrafish. J. Vis. Exp. (79), e50113, doi:10.3791/50113 (2013).

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