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在活细胞中蛋白质聚集的4D成像
JoVE Journal
Biologie
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JoVE Journal Biologie
4D Imaging of Protein Aggregation in Live Cells
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在活细胞中蛋白质聚集的4D成像

16,990 Views

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08:59 min

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April 05, 2013

DOI:

10.3791/50083-v

DOI:
10.3791/50083-v

•

08:59 min
April 05, 2013

•

1 Views
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1Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences,Hebrew University of Jerusalem

Summary

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细胞存活率依赖于蛋白质的错误折叠的及时和有效地管理。在这里，我们描述了一种方法，可视化的错误折叠的蛋白质复性，退化，或夹杂物封存在不同的潜在的命运。我们演示了如何使用的折叠式传感器，UBC9<sup> TS</sup，为的监控proteostasis和使用4D显微镜的活细胞聚集的质量控制。

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Cite this Article

Spokoini, R., Shamir, M., Keness, A., Kaganovich, D. 4D Imaging of Protein Aggregation in Live Cells. J. Vis. Exp. (74), e50083, doi:10.3791/50083 (2013).

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