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Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
JoVE Journal
Biologie
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JoVE Journal Biologie
Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

DOI:
10.3791/50044-v

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07:25 min

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March 29, 2013

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1Department of Biology,Vassar College

Kapitel

  • 00:05Titel
  • 01:28Preparing Flies for Dissection
  • 02:15Preparation of Oocytes for Imaging Using an Upright Compound Microscope
  • 04:33Live Imaging
  • 06:18Results: Live Imaging of Autofluorescent and GFP-tagged Structures
  • 07:00Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A protocol for live imaging of GFP-tagged proteins or autofluorescent structures in individual Drosophila oocytes is described.

Tags

DrosophilaOocytesLive ImagingGFP-labeled ProteinsCytoskeletonOoplasmic StreamingConfocal Microscopy

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