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Dobbelte Elektrofysiologiske Optagelser af synaptisk-evoked astrogliale og Neuronal respons i Akut hippocampusskiver
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Dobbelte Elektrofysiologiske Optagelser af synaptisk-evoked astrogliale og Neuronal respons i Akut hippocampusskiver
Kapitel
- 00:00Titel
- 03:58Dissection
- 10:23Dual Recordings of Neuronal fEPSP and Single Astrocytic Whole-cell Response
- 12:07Dual Whole-cell Recordings of Astrocyte and Neuron
- 13:53Representative Results
- 15:45Conclusion
Fremstillingen af akutte hjerneskiver fra isolerede hippocampi samt de samtidige elektrofysiologiske optagelser af astrocytter og neuroner i<em> Stratum radiatum</em> Under stimulering af Schaffer sikkerhedsstillelse beskrives. Den farmakologiske isolering af astroglial kalium og glutamat transportproteiner strømninger er blevet demonstreret.
Tags
Dual Electrophysiological RecordingsSynaptically-evoked Astroglial ResponsesNeuronal ResponsesAcute Hippocampal SlicesTripartite SynapsesAstrocytesNeuronal Activity ModulationIon ChannelsNeurotransmitter ReceptorsGliotransmittersGlutamate UptakePotassium Spatial BufferingGABA ClearanceSynaptic Activity MonitoringAlterations In Glutamate LevelsAlterations In GABA LevelsAlterations In Extracellular Potassium LevelsAstroglial Uptake ActivityBuffering CapacityPhysiopathological SituationsKnockout MiceHippocampal Slice PreparationStratum Radiatum Astrocytes IdentificationSimultaneous Neuronal And Astroglial RecordingsSynaptically-evoked Astroglial Currents Isolation
