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Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
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Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex
Kapitel
- 00:05Titel
- 02:18Anesthesia, Stereotaxic Placement and Clearance of Skull
- 03:20Cannulas, EMG Leads and EEG Leads
- 04:55Assembly of Rodent Optogenetic Stimulation System
- 06:42Measurement of EEG-defined Sleep and Lactate Concentration during Optogenetic Stimulation
- 08:19Optogenetic Stimulation: EEG-defined Sleep and Lactate Concentration
- 09:26Conclusion
A procedure is described for manipulating the activity of cerebral cortical pyramidal neurons optogenetically while the electroencephalogram, electromyogram, and cerebral lactate concentration are monitored. Experimental recordings are performed on cable-tethered mice while they undergo spontaneous sleep/wake cycles. Optogenetic equipment is assembled in our laboratory; recording equipment is commercially available.
