/
/
Granulociti-dipendente indotta da autoanticorpi pelle vescicole
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:00Titel
- 01:28Overall Scheme of the Experiment
- 02:36Preparation of Pathogenic Antibody
- 04:06Injection of Collagen VII-specific IgG into Mice
- 06:47Clinical Evaluation of Disease Severity
- 09:13Analysis of Tissue-bound and Circulation Collagen-specific Antibodies
- 10:19Representative Results
- 11:41Conclusions
Nel modello animale descritto nel nostro lavoro attuale, purificati anticorpi IgG contro un tratto di 200 aminoacidi (aa 757-967) del collagene VII vengono iniettate più volte in topi che riproducono il fenotipo vesciche così come le caratteristiche del isto-e immunopatologiche caratteristiche per la salute umana epidermolisi bollosa acquisita (EBA)<sup> 1</sup>.
Tags
Granulocyte-dependentAutoantibody-inducedSkin BlisteringAutoimmune PhenomenaSelf-toleranceAutoimmune ResponseWitebsky’s PostulatesDiagnosing Autoimmune DiseasePassive Transfer Of AutoantibodiesEpidermolysis Bullosa Acquisita (EBA)Experimental ModelsCollagen VIIBlistering PhenotypeHisto- And Immunopathological FeaturesHuman EBAWidespread DiseaseDose Of Administered Collagen VII-specific IgGGranulocyte Recruitment And ActivationInflammatory Pathway
