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优化小,解剖定义的大脑区域的DNA甲基化和基因表达分析
JoVE Journal
Neurowissenschaften
This content is Free Access.
JoVE Journal
Neurowissenschaften
Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
Please note that all translations are automatically generated.
Click here for the English version.
优化小,解剖定义的大脑区域的DNA甲基化和基因表达分析
DOI:
10.3791/3938-v
•
13:11 min
•
July 12, 2012
•
Marc Bettscheider
,
Arleta Kuczynska
,
Osborne Almeida
,
Dietmar Spengler
1
Max Planck Institute of Psychiatry
Kapitel
00:05
Titel
01:58
Programming by Early Life Adversity
02:45
Collecting Brain Tissue Punches
04:02
Nucleic Acid Extraction from Brain Punches
06:08
Bisulfite PCR
07:41
Bisulfite Sequencing
10:57
The Effect of ELS on the Expression and Methylation of AVP
12:35
Conclusion
Summary
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Automatische Übersetzung
一个精简的工作流程,研究DNA甲基化和基因表达变化后早期生活压力。从新生鼠和离散脑组织隔离母体分离开始,我们代表的协议,同时为随后的亚硫酸氢钠测序和RT-PCR分析DNA和RNA分离脑组织拳。
Tags
DNA Methylation
Gene Expression
Brain Analysis
Small Anatomically-defined Areas
Diet
Drugs
Early Life Adversity
Lasting Changes
Physiological Phenotypes
Behavioural Phenotypes
Metabolic Diseases
Cardiovascular Diseases
Mental Diseases
Environmental Programming
Gene-environment Dialogue
Histone Modifications
Mammals
CpG Methylation
Cellular Heterogeneity
Gene Expression And Methylation Correlation
Epigenetic Programming
Maternal Separation Paradigm
Micropunches Isolation
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