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Agarosegelelektroforese til adskillelse af DNA fragmenter

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Agarosegelelektroforese til adskillelse af DNA fragmenter

DOI:

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07:10 min

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April 20, 2012

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Pei Yun Lee, John Costumbrado, Chih-Yuan Hsu, Yong Hoon Kim

¹Department of Molecular, Cell, and Developmental Biology,University of California Los Angeles

Kapitel

00:05Titel
01:31Preparation of the Gel
03:21Setting up Gel Apparatus and Separating DNA Fragments
04:55Observing Separated DNA Fragments
05:43Results: Agarose Gel Electrophoresis of PCR Products
06:23Conclusion

Summary

Automatische Übersetzung

En grundlæggende protokol til adskillelse af DNA fragmenter ved hjælp af agarosegel-elektroforese er beskrevet.

Tags

Agarose Gel Electrophoresis DNA Fragments Agarose Gelation Molecular Sieving Properties Sucrose Density Gradient Centrifugation Pre-cast Wells Current Phosphate Backbone Negatively Charged Electric Field Anode Mass/charge Ratio Molecular Weight Biased Reptation DNA Movement Migration Rate

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