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RNA纯化的染色质隔离(CHIRP)
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
Chromatin Isolation by RNA Purification (ChIRP)
DOI:

11:09 min

March 25, 2012

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Kapitel

  • 00:05Titel
  • 01:25Cross-linking Cells to Preserve RNA-Chromatin Interactions
  • 02:58Cell Lysis
  • 03:37Shearing DNA by Sonication
  • 05:13Chromatin Isolation by RNA Purification (ChIRP)
  • 08:43Results: Enrichment of Target Long Noncoding RNAs by ChIRP
  • 10:34Conclusion

Summary

Automatische Übersetzung

CHIRP技术是一种新型快速映射约束力的长非编码RNA基因组网站(lncRNAs)。该方法采用特异性的反义平铺寡核苷酸允许枚举的lncRNA绑定基因组站点的优势。

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