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Kapitel
- 00:02Titel
- 01:00Overall View of Chromatin Interaction Analysis using Paired-end Tag (ChIA-PET)
- 02:15ChIP – Chromatin Immunoprecipitation
- 04:24Cell Lysis
- 05:07Nuclear Lysis
- 07:11Fragmentation of Chromatin
- 08:26Washing, Preclearing, and Coating of Antibody to Beads
- 10:08Chromatin Immunopreciptation
- 11:20Washing and Elution
- 11:48ChIA-PET – Chromatin Interaction Analysis using Paired-end Tag Sequencing
- 12:29End-blunting of ChIP DNA Fragments
- 13:33Ligation of Biotinylated Half-linkers to ChIP DNA
- 16:02Elution and Proximity Ligation of ChIP DNA Fragments
- 18:08Immobilization of ChIA-PET DNA to Streptavidin Beads
- 20:47Conclusion
Tags
Chromatin Interaction AnalysisPaired-End Tag SequencingChIA-PETMapping Chromatin InteractionsTranscription RegulationThree-dimensional StructuresHigher-order ConformationsNuclear SpacesGene PromotersRegulatory ElementsTranscription FactorsRegulatory SequencesNetwork Of Transcription RegulationCoordinationChromatin ImmunoprecipitationChIPChromatin FragmentsProximity LigationCovalent LinksDNA FragmentsChromatin ComplexesMmeI Restriction Enzyme SitesHalf-linkersPET Constructs
