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의 RNA 격리 녹농균 Murine 위장관을 Colonizing
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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의 RNA 격리 녹농균 Murine 위장관을 Colonizing

18,782 Views

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08:52 min

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September 28, 2011

DOI:

10.3791/3293-v

DOI:
10.3791/3293-v

•

08:52 min
September 28, 2011

•

6 Views
, , ,
1Department of Pediatrics,University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pediatrics and Microbiology,University of Texas Southwestern Medical Center

Summary

Automatically generated

의 RNA 격리에 대한 신뢰할 수있는 방법<em> 녹농균</em> murine cecums에서 발견한 것은 설명되어 있습니다. 회수 RNA는 다음 qPCR, 전사 프로 파일링, 및 RNA Seq 실험을위한 충분한 수량과 품질입니다. 이 기술은 다른 장내 미생물의 RNA 분리에 대해 적용할 수 있습니다.

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Cite this Article

Lopez-Medina, E., Neubauer, M. M., Pier, G. B., Koh, A. Y. RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract. J. Vis. Exp. (55), e3293, doi:10.3791/3293 (2011).

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