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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Kapitel
- 00:05Titel
- 03:54Specimen Collection and Preservation
- 05:04DNA Extraction
- 06:01Verification of DNA Quality and Quantity
- 06:47Genotyping PCR
- 09:17Results from the Electrophoresis of Genotyping PCR Products
- 11:02Conclusion
We provide a cost-effective and rapid molecular genotyping protocol that employs variety-specific PCR primers that target DNA sequence differences within the chloroplast trnL-F spacer region to differentiate between varieties of Imperata cylindrica (cogongrass) that cannot be distinguished by morphology alone. These varieties include the federally listed noxious weed, cogongrass and closely-related, wide-spread ornamental variety, I. cylindrica var. koenigii (Japanese blood grass).
Tags
PCR-based Genotyping MethodWild-typeOrnamental VarietiesImperata CylindricaCogongrassInvasive PlantsNative Plant SpeciesOrnamental VarietyI. Cylindrica Var. KoenigiiRubraRed BaronJapanese Blood GrassJBGSterile And NoninvasiveViable Seed ProductionGreen Invasive FormMorphology IdentificationPlant TaxonomistsSequence ComparisonsCoding RegionsVariable RegionsNuclear DNAChloroplast DNA
