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多光子成像在口腔鳞状细胞癌原位小鼠模型的肿瘤细胞的侵袭
JoVE Journal
Medizin
This content is Free Access.
JoVE Journal
Medizin
Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
Please note that all translations are automatically generated.
Click here for the English version.
多光子成像在口腔鳞状细胞癌原位小鼠模型的肿瘤细胞的侵袭
DOI:
10.3791/2941-v
•
12:03 min
•
July 25, 2011
•
Amanda Gatesman Ammer
,
Karen E. Hayes
,
Karen H. Martin
,
Lingqing Zhang
,
George A. Spirou
,
Scott A. Weed
1
Department of Neurobiology and Anatomy, Program in Cancer Cell Biology
,
Mary Babb Randolph Cancer Center, West Virginia University
,
2
Sensory Neuroscience Research Center
,
West Virginia University
,
3
Departments of Otolaryngology and Physiology, Center for Neuroscience
,
West Virginia University
Kapitel
00:05
Titel
01:46
Cell Lines, Vector Construction, and Lentiviral Production
03:19
Production of Head and Neck Cell Lines with Stable LifeAct-mCherry Expression
04:30
Orthotopic Tumor Xenograft Formation
05:56
Preparation of Mouse Tongues for Ex-vivo Imaging
06:27
Imaging of Tongue Tumors with Two-photon Microscopy and Image Analysis
09:55
Representative Tumor Images
11:04
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
产生的口腔癌和肿瘤浸润的定量监测的小鼠模型,通过多光子显微镜标记细胞内的舌头所涉及的技术的一个全面的概述。该系统可以作为一个有用的平台和反侵入性化合物的分子评估药物疗效。
Tags
Multi-photon Imaging
Tumor Cell Invasion
Orthotopic Mouse Model
Oral Squamous Cell Carcinoma
Head And Neck Cancer
Metastatic Risk
Patient Management Strategies
Anti-tumor Therapeutics
Disseminated Tumor Cells
Conventional Histology
In Vivo Bioluminescent Methods
Early Tumor Cell Invasion
Three Dimensions Visualization
Squamous Cell Carcinoma Of The Tongue (SCOT)
Two-photon Imaging
Lingual Tumor Spread
OSC-19 Head And Neck Tumor Cell Line
F-actin Binding Peptide LifeAct
MCherry Fluorescent Protein
Article
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