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Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
JoVE Journal
Medizin
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JoVE Journal Medizin
Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging

Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging

DOI:
10.3791/2808-v

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08:04 min

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June 21, 2011

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, , , ,
1Department of Medical Biophysics,University of Western Ontario, 2London Regional Cancer Program,London Health Science Centre, 3Department of Pathology,Vanderbilt University , 4Translational Prostate Cancer Research Group,London Health Science Centre

Kapitel

  • 00:05Titel
  • 00:21Subscription
  • 01:38Inoculation of Tumors into Avian Embryo CAM
  • 03:10Preparation of Nanoparticles
  • 03:52Intravenous Injection of Fluorescent Nanoparticles
  • 05:00Real Time Intravital Imaging and Quantification
  • 06:47Uptake of CPMV-PEG into a CAM Tumor
  • 07:35Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We present a novel approach to quantify nanoparticle localization in the vasculature of human xenografted tumors using dynamic, real-time intravital imaging in an avian embryo model.

Tags

Nanoparticle UptakeTumorsIntravital ImagingXenograft ModelHigh-resolution ImagingChicken Embryo ModelTumor VasculatureNanoparticle AnalysisCowpea Mosaic Virus (CPMV)Near-infrared Fluorescent DyesPolyethylene Glycol Polymers (PEG)Intravenous Administration

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