Journal
/
Neurowissenschaften
/
Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures
JoVE Journal
Neurowissenschaften
This content is Free Access.
JoVE Journal Neurowissenschaften
Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

DOI:
10.3791/2583-v

•

06:34 min

•

April 21, 2011

•

, , ,
1Dept. of Anatomy, Physiology and Genetics,Uniformed Services University, 2Neuroscience Program,Uniformed Services University

Kapitel

  • 00:05Titel
  • 01:12Reagent Preparation for 3D Tissue Culture
  • 02:22Brain Tissue Dissection and Explant Collection
  • 03:223D Tissue Culture Setup
  • 04:36Live Imaging of 3D Tissue Culture
  • 05:183D Culture Cell Migration Dynamics and Image Analysis
  • 05:59Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Time lapse imaging of 3D tissue culture allows studying migratory behavior of individual cells originating from ganglionic eminence in reaction to fractionated protein extract from cerebral cortex.

Tags

Migratory BehaviorCellsGanglionic Eminence CulturesCerebral CortexExcitatory NeuronsInhibitory NeuronsSubcortical SourcesSpatiotemporal ControlCytoarchitectureConnectivityProliferative ZonesNewborn Ferrets3 Dimensional Culture ArrangementBD Matrigel MatrixGE ExplantsTested ProteinsFluorescent Latex Retrobeads IXLive Imaging

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Selective Tracing of Auditory Fibers in the Avian Embryonic Vestibulocochlear Nerve

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

Assays to Detect UV-reflecting Structures and Determine their Importance in Mate Preference using the Sailfin Molly Poecilia latipinna

Quantification of Drosophila Grooming Behavior

In Vivo Optical Calcium Imaging of Learning-Induced Synaptic Plasticity in Drosophila melanogaster

Measuring and Manipulating Functionally Specific Neural Pathways in the Human Motor System with Transcranial Magnetic Stimulation

Read Article