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使用一种基因编码的钙传感器来监测细胞凋亡过程中线粒体钙水平的动态变化
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April 01, 2011
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本协议描述了线粒体钙通量实时荧光成像测量方法。该方法采用一个基于循环permutated YFP的双激励比例的钙传感器选择性表达在线粒体（比例pericam吨）的优势。
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Akimzhanov, A. M., Boehning, D. Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor. J. Vis. Exp. (50), e2579, doi:10.3791/2579 (2011).
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