/
/
Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
35,250 Views
•
18:11 min
•
November 16, 2010
DOI:
Summary
Automatically generated
This protocol details how to quantify synapse number both in dissociated neuronal culture and in brain sections using immunocytochemistry. Using compartment-specific antibodies, we label presynaptic terminals as well as sites of postsynaptic specialization. We define synapses as points of colocalization between the signals generated by these markers.
Explore More Videos
QuantifySynapsesImmunocytochemistry-based AssaySynapse NumberNeurowissenschaftenMolecular CuesObjective ApproachesSynaptic ConnectivitySample FixationDissociated Neuronal CultureBrain SectionsImmunocytochemistryCompartment-specific AntibodiesPresynaptic TerminalsPostsynaptic SpecializationColocalizationSignalsMarkersPuncta AnalyzerImageJ Analysis Software PlatformSynapse AssayCulture Preparation
Read Article
Cite this Article
Ippolito, D. M., Eroglu, C. Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number. J. Vis. Exp. (45), e2270, doi:10.3791/2270 (2010).
Copy