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Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
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JoVE Journal Neurowissenschaften
Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

DOI:
10.3791/2184-v

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08:37 min

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November 18, 2010

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,
1Biotechnik,University of California, San Diego, 2La Jolla Bioengineering Institute

Kapitel

  • 00:00Titel
  • 02:05Preparations for Intravital Microscopy
  • 03:34Measuring Vessel Diameter and RBC Velocity by Intravital Microscopy
  • 06:13Perfusion Assessment and Analysis of Leukocyte Adherence by Intravital Microscopy
  • 07:08Representative Results of Intravital Microscopy
  • 08:03Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Intravital microscopy to follow temporal and spatial hemodynamic and inflammatory events in the pial microcirculation.

Tags

Intravital MicroscopyMouse BrainMicrocirculationClosed Cranial WindowAcute ConditionsChronic ConditionsPhysiological ConditionsPathophysiological ConditionsIn Vivo Fluorescence MicroscopyPial MicrocirculationReal-time RecordingEpi IlluminationFluorescence IlluminationVessel DiametersRed Blood Cell VelocitiesRBC Velocity MeasurementCross-correlationFluorescent-labeled ErythrocytesLeukocyte AdherencePlatelet AdherencePerfusion AssessmentVascular Leakage AssessmentFluorescence-labeled MarkersAlbumin-FITC MarkerAnti-CD45-TxR AntibodiesVideo RecordingPlasmodium Berghei ANKA InfectionMicrocirculatory Dysfunctions

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