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与预先定义的T细胞受体特异性Transnuclear小鼠对弓形虫获得通过体细胞核移植
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September 30, 2010
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我们证明，在这里，通过体细胞核移植（SCNT）后生重新编程可以作为一个工具来生成与预先定义的T细胞受体（TCR）特异性的小鼠模型使用。这些transnuclear小鼠表达内源性启动子的控制下的内源性轨迹相应的TCR。
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Transnuclear MicePre-defined T Cell Receptor SpecificitiesToxoplasma GondiiSCNTLymphocytesGenetic RecombinationAntigen-specific T Cell ReceptorT Cell DevelopmentT Cell FunctionLong-term CultureAntigen StimulationHigh AffinityRandom Genomic IntegrationExpression LevelKineticsEpigenetic ReprogrammingSomatic Cell Nuclear TransferEmbryonic Stem CellsMouse ModelsEndogenous LociDisease Models
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Kirak, O., Frickel, E., Grotenbreg, G. M., Suh, H., Jaenisch, R., Ploegh, H. L. Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT. J. Vis. Exp. (43), e2168, doi:10.3791/2168 (2010).
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