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Electroeluting DNA Fragments
JoVE Journal
Biologie
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JoVE Journal Biologie
Electroeluting DNA Fragments

Electroeluting DNA Fragments

DOI:
10.3791/2136-v

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06:13 min

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September 05, 2010

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, , ,
1Department of Genetics and Molecular Biology ,Research and Advanced Studies Center of National Polytechnic Institute

Kapitel

  • 00:00Titel
  • 00:08Introduction
  • 00:53Electrophoresis and Excision
  • 01:44Electroelution
  • 02:57Purification and Quantification
  • 05:31Summary

Summary

Automatische Übersetzung

Automatische Übersetzung

This procedure allows the purification of DNA fragments with high yield.

Tags

Electroeluting DNA FragmentsPurified DNA FragmentsMolekularbiologieElectrophoresisAgarose GelAcrylamide GelBinding And ElutionGlass ParticlesSilica ParticlesDEAE-cellulose MembranesCrush And Soak MethodElectroelutionCommercial Purification KitsLaboratoryClean DNASequencingRadiolabelingEnzymatic RestrictionEnzymatic ModificationCloningSample WellsElectroeluterCurrentCushion SaltEthanol Precipitation

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