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Sample Mounting for Light-Sheet Fluorescence Microscopy and Stripping for Multi-Round Labeling of the Same Specimens

JoVE Journal
Neurowissenschaften

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JoVE Journal Neurowissenschaften

Sample Mounting for Light-Sheet Fluorescence Microscopy and Stripping for Multi-Round Labeling of the Same Specimens

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Sample Mounting for Light-Sheet Fluorescence Microscopy and Stripping for Multi-Round Labeling of the Same Specimens

78 Views

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01:58 min

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January 26, 2024

DOI:

10.3791/200807-v

DOI:

10.3791/200807-v

•

01:58 min

January 26, 2024

•

5 Views

Danila Di Meo*¹, Josephine Ramazzotti*¹, Marina Scardigli*^1,2, Franco Cheli, Luca Pesce³, Niamh Brady, Giacomo Mazzamuto^4,5, Irene Costantini^4,6, Francesco S. Pavone^4,5

¹European Laboratory for Non-linear Spectroscopy (LENS),University of Florence, ²Division of Physiology, Department of Experimental and Clinical Medicine,University of Florence, ³Department of Physics,University of Pisa, ⁴National Research Council – National Institute of Optics (CNR-INO), ⁵Department of Physics and Astronomy,University of Florence, ⁶Department of Biology,University of Florence

Tags

Sample Mounting Light-sheet Fluorescence Microscopy Multi-round Labeling Steel Spacer Quartz Cover Slip Silicone Glue TDE PBS Clearing Solution Broca’s Area NeuN Somatostatin Claritin Interneurons Light Sheet Fluorescence Microscopy Neuronal Markers Maximum Intensity Projection

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