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Journal
/
Biologie
/
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
Processing Murine Small Intestine for Crypt Isolation
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Processing Murine Small Intestine for Crypt Isolation
Processing Murine Small Intestine for Crypt Isolation
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/200006-v
•
01:35 min
•
June 02, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
Crypt Isolation
Small Intestine
Murine
PBS-antibiotics
Euthanized Animal
Manual Washing
Pipette
Laboratory Scissors
Tweezers
Petri Dish
50-milliliter Tube
Embed
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Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research
Processing Murine Small Intestine for Crypt Isolation
Crypt Isolation from Murine Small Intestine
Organoid Culture from a Single Intestinal Stem Cell
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