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对活细胞成像的SNAP - Tag融合蛋白的荧光标记的COS - 7
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07:38 min
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May 17, 2010
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Summary
Automatically generated
SNAP标签和剪辑标签蛋白标签制度，包括荧光染料分子，使特定的共价结合，一个活细胞中的兴趣的蛋白质。一旦克隆和表达，标记蛋白可用于许多下游应用的各种基材，而无需再次进行克隆。
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Fluorescent LabelingCOS-7SNAP-tagFusion ProteinsLive Cell ImagingProtein Labeling SystemsCovalent AttachmentFluorescent DyesProtein Of InterestImaging InstrumentationCloningExpressionDownstream ApplicationsSNAP-tag SubstrateHuman O6-alkylguanine-DNA-alkyltransferase (hAGT)DNA Repair ProteinBenzyl LinkerLabeling ReactionCLIP-tagOrthogonal Labeling
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Cite this Article
Provost, C. R., Sun, L. Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging. J. Vis. Exp. (39), e1876, doi:10.3791/1876 (2010).
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