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一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
Please note that all translations are automatically generated.
Click here for the English version.
一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析
DOI:
10.3791/1173-v
•
15:04 min
•
May 05, 2009
•
Janet L Anderl
,
Stella Redpath
,
Andrew J Ball
1
Bioscience Division, High Content Analysis Research and Development
,
Millipore Inc
Kapitel
00:05
Titel
00:48
Introduction
01:28
Cell Preparation for Neurotoxicity Screens
02:57
Cell Fixation and Immunofluorescent Staining
06:07
HCS Imaging, Analysis, and Results
14:15
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
本文介绍了一种新的协议和试剂,为敏感的神经毒性作用的化合物和治疗联合培养的神经元和星形胶质细胞使用高含量的分析测量设计。结果表明,高含量的分析,代表着一个令人兴奋的新技术,神经毒性评估。
Tags
Neuronal And Astrocyte Co-culture Assay
High Content Analysis
Neurotoxicity
Drug Safety Evaluation
Environmental Protection
Neurodegenerative Diseases
Alzheimer’s Disease
Parkinson’s Disease
Neuronal Screening
βIII-tubulin Labeling
High-throughput
Quantitative Measurements
Neuronal Number
Neurite Count
Neurite Length
Neurotoxic Effects
Astrocytes Role
Central Nervous System Homeostasis
Neuroprotection
Neurodegradation
GFAP Upregulation
Article
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