JOVE JOURNAL

Biochemie

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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Cristina Lento¹, Shaolong Zhu¹, Kerene A. Brown¹, Ruth Knox¹, Peter Liuni¹, Derek J. Wilson^1,2,3

¹Department of Chemistry,York University, ²The Centre for Research in Mass Spectrometry,York University, ³The Centre for Research on Biomolecular Interactions,York University

Journal (Biochemistry)

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

Xavier Gallart-Palau*¹, Aida Serra*¹, Siu Kwan Sze¹

¹Division of Structural Biology and Biochemistry, School of Biological Sciences,Nanyang Technological University

Journal (Biochemistry)

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Cory A. Bontrager¹, Tanner J. Geibel¹, George A. Lengyel¹

¹Department of Chemistry,Slippery Rock University

Journal (Biochemistry)

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Mercedes Pardo¹, Daniel Bode¹, Lu Yu¹, Jyoti S. Choudhary¹

¹Proteomic Mass Spectrometry,Wellcome Trust Sanger Institute

Journal (Biochemistry)

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

David J. Samuels¹, Zhe Wang², Kyu Y. Rhee^2,3, Shaun R. Brinsmade¹

¹Department of Biology,Georgetown University, ²Division of Infectious Diseases,Weill Cornell Medical College, ³Department of Medicine,Weill Cornell Medical College

Journal (Biochemistry)

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Andrew Baxter¹, Emmanuel Minet¹

¹Research and Development,British American Tobacco

Journal (Biochemistry)

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Christopher J. A. Warner¹, Subrata Dutta¹, Alejandro R. Foley¹, Jevgenij A. Raskatov¹

¹Department of Chemistry,University of California, Santa Cruz

Journal (Biochemistry)

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Stamatios Liokatis¹

¹Department of Structural Biology,Leibniz Institute of Molecular Pharmacology (FMP-Berlin)

Journal (Biochemistry)

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Hyelee Lee¹, Shih-Yuan Liu¹

¹Department of Chemistry,Boston College

Journal (Biochemistry)

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Eric D. Routh*¹, Steven D. Creacy*², Peter E. Beerbower³, Steven A. Akman⁴, James P. Vaughn¹, Philip J. Smaldino³

¹Department of Cancer Biology,Wake Forest School of Medicine, ²YX Genomics, ³Department of Biology,Ball State University, ⁴Department of Hematology and Oncology,Roper St. Francis Hospital

Journal (Biochemistry)

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Ramakrishnan B. Kumar¹, Lin Zhu², Hans Hebert^1,2, Caroline Jegerschöld^1,2

¹Department of Biosciences and Nutrition,Karolinska Institutet, ²School of Technology and Health,KTH Royal Institute of Technology

Journal (Biochemistry)

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Robyn M. Johnston¹, Anne W. Sylvester², Michael J. Scanlon¹

¹Plant Biology Section, School of Integrative Plant Science,Cornell University, ²Department of Developmental Genetics,University of Wyoming

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