An Assay for Analyzing Test β-Glucan as an Immunotherapy Strategy against Brain Cancer Cells

Published: May 31, 2024

Abstract

Source: Folgado-Dorado, C. et al., Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma. J. Vis. Exp. (2023)

This video demonstrates an in vitro assay utilizing β-glucans to activate microglial cells for the production of superoxide radicals, a reactive oxygen species. In brain cancer cells, the generated reactive oxidants actively suppress tumor cell proliferation and induce cell death.

Protocol

In vitro study of β-glucan-induced microglia stimulation 1. Cell culture of mouse glioblastoma and microglia cells in 8-well chamber slides NOTE: This protocol is specific for GL261 (glioblastoma) and BV2 (microglia) cell lines. However, with slight modifications, these steps could potentially be used to study other cancer and immune cell lines. Prepare Dulbecco's modified Eagle's medium (DM…

Offenlegungen

The authors have nothing to disclose.

Materials

8-well chamber slides Thermo Fisher, USA 171080
Dulbecco's modified Eagle's medium, Gluta MAXTM Gibco, Life Technologies, Carlsbad, CA, USA 10564011
Extenda (α- Amylase/Glucoamylase) Novozymes, Denmark
Fetal bovine serum Gibco, Life Technologies, Carlsbad, CA, USA A4736301
Phosphate-buffered saline Gibco, Life Technologies, Carlsbad, CA, USA 1010-015
Penicillin/streptomycin Sigma-Aldrich, St. Louis P4458
Incubator Eppedorf Galaxy 170S

Tags

This article has been published
Video Coming Soon
Keep me updated:

.

Diesen Artikel zitieren
An Assay for Analyzing Test β-Glucan as an Immunotherapy Strategy against Brain Cancer Cells. J. Vis. Exp. (Pending Publication), e22276, doi: (2024).

View Video