Intracisternal Delivery of Meningococci into Mouse Models to Induce Meningococcal Meningitis

Published: August 31, 2023

Abstract

Source: Pagliuca, C., et al., Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery. J. Vis. Exp. (2019)

In this video, we demonstrate the intracisternal delivery of Neisseria meningitidis into a mouse model to generate meningitis infection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Intra-cisternal injection of mice NOTE: The entire procedure is performed in the laminar flow cabinet to maintain aseptic conditions. House laboratory mice (8-week-old, female Balb/c) under specific pathogen-free conditions. Provide food pellets and water ad libitum….

Offenlegungen

The authors have nothing to disclose.

Materials

Alarm-Thermometer TESTO 9000530 Device
BD Micro Fine syringe BD 320837 U-100 Insulin
BD Plastipak syringe 1ml 25GA 5/8in BD 300014 05x16mm
BD Plastipak syringe 5ml BD 308062 07 x 30mm
BIOHAZARD AURA B VERTICAL LAMINAR FLOW CABINET Bio Air s.c.r.l. Aura B3 Device
C150 CO2 Incubator Binder 9040-0078 Device
Diete Standard Certificate Mucedola s.r.l. 4RF21 Food pellet for animal
Dumont Hp Tweezers 5 Stainless Steel F.S.T. by DUMONT AGT5034 0,10 x 0,06 mm tip
Electronic Balance Gibertini EU-C1200 Max 1200g, d=0.01g, T=-1200g
Iron dextran solution Sigma-Aldrich D8517-25ML
Ketamine Intervet
Microbiological Safety Cabinet BH-EN and BHG Class II Faster BH-EN 2004
Microcentrifuge tubes 1.5ml BRAND PP780751 Screw cap PP, grad
Mouse Handling Forceps F.S.T. 11035-20 Serrated rubber; Gripping surface:15 x 20 mm
Natural Latex Gloves Medica M101
Touch N Tuff disposible nitrile gloves Ansell 92-500
Xylazine Intervet

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Intracisternal Delivery of Meningococci into Mouse Models to Induce Meningococcal Meningitis. J. Vis. Exp. (Pending Publication), e21602, doi: (2023).

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