PDMS precursor |
Dow corning |
184 SIL ELAST KIT 3.9KG |
10:1 ratio of base and curing agent |
VWR gravity convection oven |
VWR |
414005-128 |
PDMS precursor to be cured in 90 deg. |
100mm petri dish |
VWR |
89000-324 |
Fabrication of PDMS Supporting layer |
Harris Uni-core puncher |
Sigma-aldrich |
WHAWB100076 |
2mm diameter or other depending on the tubing size |
Air plasma machine |
Femto Science |
Cute |
Surface plasma treatment for PDMS device to bottom base. |
2" x 3" glass slide |
TED PELLA, INC. |
2195 |
To support PDMS device |
Masterflex spooled platinum-cured silicone tubing, L/S 14 |
Cole-Parmer |
EW-96410-14 |
Tubing for microfluidics and peristlatic pump |
1/16 inch Luer connector, male |
Harvard apparatus |
PC2 72-1443 |
Connector for fluid guide |
50mL Falcon tube |
Corning |
21008-940 |
Sample collection & preparation |
Phosphate-Buffered Saline, 1X Without Calcium and Magnesium |
Corning |
45000-446 |
Buffer solution to dilute sample |
Halyard Closed suction Catheter, Elbow, 14F/ channel 4.67mm |
HALYARD HEALTH |
22113 |
Tracheal seceation suction catheter |
0.9% Sterile Normal saline, 10mL pre-filled syringe |
BD PosiFlush |
NHRIC: 8290-306547 |
For tracheal seceation collection from the patients |
SecurTainer™ III Specimen Containers, 20mL |
Simport |
1176R36 |
Sterile sputum (airway secretion) collection container |
Syringe with Luer-Lok Tip, 10mL |
BD |
BD309604 |
To pipette homogenize the mucus sample and reach the bottom of sample tube |
BD Blunt Fill Needle, with BD Luer-Lok Tip |
BD |
|
To pipette homogenize the mucus sample and reach the bottom of sample tube |
40µm nylon cell strainer |
Falcon |
21008-949 |
To remove large chunk or blood clots, which can block the microfluidics access hole or the channel. |
Peristaltic pump (Masterflex L/S Digital Drive) |
Cole-Parmer |
HV-07522-30 |
Operation of microfluidics |