Mosquito Feeding Assay: A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes

Published: April 30, 2023

Abstract

Source: Brito-Sierra, C. A. et al., Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes. J. Vis. Exp. (2019).

This video demonstrates a blood-feeding assay to evaluate the mortality and fecundity of test compounds with novel modes of action on female mosquitoes. This technique helps in screening new classes of insecticides to kill the resistant population of mosquitoes and control disease spread.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Adult Blood-Feeding Assay (Single Point Dose or Dose-Response Assay) Collect approximately 150, 4-5-day old adult female mosquitoes and transfer to a separate cage. Repeat to obtain 6 cages if performing a dose-response assay. Remove the source of sugar 1-24 h prior to the feeding assay. Prepare a stock solution of chemistry as …

Offenlegungen

The authors have nothing to disclose.

Materials

Insecticide susceptible mosquito strains Malaria Research and Reagent Reference Repository https://www.beiresources.org/ MR4Home.aspx; Recommended strains: Aedes aegypti Liverpool (LVP) strain, Anopheles gambiae Kisumu (KISUMU1) strain and Culex quinquefasciatus Johannesburg (JHB) strain
Bifenthrin Sigma-Aldrich CAS: 51529-01-2 Synthetic pyrethorid used as positive control
Defibrinated rabbit blood Hemostat DRB0100 Blood source for adapted mosquitoes to artificial feeding
Hemotek membrane feeding system Hemotek Ltd Serial no. 1303 Used for feeding mosquitoes
Transfer pipettes Fisher Scientific 13-711-7M U
Stereo microscope Olympus SZ6045 Used to score larval assays and perform micro-applications
Tweezers (fine) Fontax Used to handle adult mosquitoes
Paper cups Dixie Consumer Products LLC. PF15675/13D Used to keep adult mosquitoes in adult topical assays
Advantage rubber bands Alliance Rubber Co. Used to seal the paper cups

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Mosquito Feeding Assay: A Method to Evaluate Test Compound Effects on Mortality and Fecundity of Adult Female Mosquitoes. J. Vis. Exp. (Pending Publication), e21171, doi: (2023).

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