In Vivo Biomechanical Testing of Nerve: A Procedure for Biomechanical Analysis of Brachial Plexus Nerve Injury in a Neonatal Pig Model
Published: April 30, 2023
Abstract
Source: Singh, A., et al. Methods for In Vivo Biomechanical Testing on Brachial Plexus in Neonatal Piglets. J. Vis. Exp. (2019).
In this video, we demonstrate the biomechanical testing of the brachial plexus nerve in a pig model to measure the threshold tensile strength of the nerve when subjected to stretching. This technique helps in determining the structural and mechanical properties of the nerve.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Biomechanical Testing Set-up of the biomechanical testing device NOTE: A custom-built mechanical testing device was designed and fabricated to perform in vivo stretch of the brachial plexus (BP) nerve (Figure 1). Attach the base of the set-up to a cart. <l…
Representative Results
Figure 1: Details of in vivo mechanical testing device including the actuator, load cell, and clamp. Figure 2: Markers placed over the BP segments to record strains sustained by the tissue during the stretch. <p class="jove_content" fo:keep…
Offenlegungen
The authors have nothing to disclose.
Materials
| Omega Subminature Tension & Compression Load Cell | Omega | LCM201-200N | 200N load cel |
| Basler acA640-120uc camera | Basler | acA640-120uc | – |
| Feedback Linear Actuator | Progressive Automations | PA-14P | 10" stroke, 150lb force, 15mm/s speed |
| Motion Tracking Software | Kinovea | N/A | Open Source |
| Proramming Software – MATLAB | Mathworks | N/A | version 2018A |
| Scissors | Fine Science Tools Inc | 14094-11 or 14060-09 | – |
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In Vivo Biomechanical Testing of Nerve: A Procedure for Biomechanical Analysis of Brachial Plexus Nerve Injury in a Neonatal Pig Model. J. Vis. Exp. (Pending Publication), e20924, doi: (2023).