Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue

Published: April 30, 2023

Abstract

Source: Ou, Q. et. al., Slicing and Culturing Pig Hearts under Physiological Conditions. J. Vis. Exp. (2020)

This video demonstrates a vibratome-based procedure to generate viable, ultra-thin sections of pig heart. The prepared tissue slices can be cultured and used as model systems for pharmacological testing.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Pig Heart Tissue Slicing Set up the tissue bath on the vibratome, add ice to the tissue bath cooling jacket, then add Tyrode's solution into the tissue bath. Set up 1 L plastic jar to collect the disposal of melted ice from the tissue bath cooling jacket. For 1 L of the slicing Tyrode's solution, mix 3 g/L 2,3-butanedione monoxi…

Offenlegungen

The authors have nothing to disclose.

Materials

Agarose Bioline USA BIO-41025
2,3-Butanedione monoxime (BDM) Fisher AC150375000
Calcium Chloride (CaCl2) Fisher C79-500
Ceramic Blades for Vibrating Microtome Campden Instruments 7550-1-C
D-Glucose Fisher D16-1
Disposable Scalpel #20 Biologyproducts.com DS20X
Falcon Cell Strainers, Sterile, Corning VWR 21008-952
Heparin sodium salt Sigma-Aldrich H3149-50KU
HEPES Fisher BP310-1
Histoacryl BLUE Tissue glue Amazon https://www.amazon.com/HISTOACRYL-FLEXIBLE-1051260P-Aesculap-Adhesive/dp/B074WB5185/
Iris Spring scissors Fisher NC9019530
Iris Straight Scissors Fisher 731210
Magnesium Chloride (MgCl2) Fisher M33-500
Oxygen regulator Praxair
Oxygen tanks  Praxair
Plastic Pasteur pipettes Fisher 13-711-48
Potassium Chloride (KCl) Fisher AC193780010
Razor rectangle blades Fisher 12-640
Retractable scalpels Fisher 22-079-716
Sodium Bicarbonate (NaHCO3) Fisher AC217125000
Sodium Chloride (NaCl) Fisher AC327300010
Vibrating Microtome Campden Instruments 7000 SMZ-2

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Diesen Artikel zitieren
Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue. J. Vis. Exp. (Pending Publication), e20837, doi: (2023).

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