Roux-en-Y Gastric Bypass Procedure: A Bariatric Surgical Procedure for Gastric Resectioning in Murine Model
Published: April 30, 2023
Abstract
Source: Ayer, A., et al., Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice. J. Vis. Exp. (2017).
This video describes a bariatric surgery-Roux-en-Y procedure. This involves the cutting of the jejunum- the middle portion of the intestine- to generate two intestinal limbs. The biliary limb is attached to the existing intestinal loop and the alimentary limb is attached to the stomach, creating a gastric bypass. The gastric bypass created here helps to decrease the nutrient uptake and efficiently reduces body weight.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. General Preoperative Preparation Fast the mice for 6 h before the surgery. Gel diet food was given 3 days before surgery and exclusively one day before surgery. Induce anaesthesia in a chamber with 5% isoflurane (0.4 L/min) and O2 (dioxygen; 0.4 L/min). Perform a toe-pinch test to confirm that the anesth…
Representative Results
Figure 1: Roux-en-Y bypass Procedure. (A) Preoperative preparation. (B) Median laparotomy. (C) Externalization of the small intestine. (D) Biliopancreatic and alimentary limbs ligatures. (E) Small intestine section between the ligatures. (F) Intestinal limb positioning for the jejuno-jejunos…
Offenlegungen
The authors have nothing to disclose.
Materials
| Drugs | |||
| High Fat diet | DIO diet | Safe | |
| Isoflurane | Forane | Baxter | |
| Buprenorphin | Buprecare | Animalcare | |
| Marbofloxacine | Marbocyl | Vetoquinol | |
| Ammonium iron citrate, vitamins PP-B12 |
Fercobsang | Vetoquinol | |
| Vitamins A-D3-E-K-B | Vita Rongeur | Virbac | |
| NaCl 0.9% | NaCl 0,9% | ||
| Povidone solution | Betadine Scrub | Betadine | |
| Povidone solution | Betadine Solution | Betadine | |
| Carboptol 980 NF | Ocrygel | TVM | |
| Sutures | |||
| Prolene® | 8.0, 6,5 mm | Ethicon | |
| Prolene® | 5.0, 13 mm | Ethicon | |
| Surgical equipments | |||
| Scissors | FST | ||
| Needle holder | Olsen-Hegar | FST | |
| Micro scissors | Vannas | FST | |
| Micro forceps | Graefe | FST | |
| Micro forceps curved | Graefe | FST | |
| Curved micro needle holder | Castroviejo | FST | |
| Hemostatic collagen compress | Pangen | Urgo | |
| Absorbent underpads | VWR | ||
| Specific equipments | |||
| Stereo microscope | MZ6 | Leica |
Tags
Diesen Artikel zitieren
Roux-en-Y Gastric Bypass Procedure: A Bariatric Surgical Procedure for Gastric Resectioning in Murine Model. J. Vis. Exp. (Pending Publication), e20815, doi: (2023).