Roux-en-Y Gastric Bypass Procedure: A Bariatric Surgical Procedure for Gastric Resectioning in Murine Model

Published: April 30, 2023

Abstract

Source: Ayer, A., et al., Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice. J. Vis. Exp. (2017).

This video describes a bariatric surgery-Roux-en-Y procedure. This involves the cutting of the jejunum- the middle portion of the intestine- to generate two intestinal limbs. The biliary limb is attached to the existing intestinal loop and the alimentary limb is attached to the stomach, creating a gastric bypass. The gastric bypass created here helps to decrease the nutrient uptake and efficiently reduces body weight.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. General Preoperative Preparation Fast the mice for 6 h before the surgery. Gel diet food was given 3 days before surgery and exclusively one day before surgery. Induce anaesthesia in a chamber with 5% isoflurane (0.4 L/min) and O2 (dioxygen; 0.4 L/min). Perform a toe-pinch test to confirm that the anesth…

Representative Results

Figure 1: Roux-en-Y bypass Procedure. (A) Preoperative preparation. (B) Median laparotomy. (C) Externalization of the small intestine. (D) Biliopancreatic and alimentary limbs ligatures. (E) Small intestine section between the ligatures. (F) Intestinal limb positioning for the jejuno-jejunos…

Offenlegungen

The authors have nothing to disclose.

Materials

Drugs
High Fat diet DIO diet Safe
Isoflurane Forane Baxter
Buprenorphin Buprecare Animalcare
Marbofloxacine Marbocyl Vetoquinol
Ammonium iron citrate, vitamins
PP-B12
Fercobsang Vetoquinol
Vitamins A-D3-E-K-B Vita Rongeur Virbac
NaCl 0.9% NaCl 0,9%
Povidone solution Betadine Scrub Betadine
Povidone solution Betadine Solution Betadine
Carboptol 980 NF Ocrygel TVM
Sutures
Prolene® 8.0, 6,5 mm Ethicon
Prolene® 5.0, 13 mm Ethicon
Surgical equipments
Scissors FST
Needle holder Olsen-Hegar FST
Micro scissors Vannas FST
Micro forceps Graefe FST
Micro forceps curved Graefe FST
Curved micro needle holder Castroviejo FST
Hemostatic collagen compress Pangen Urgo
Absorbent underpads VWR
Specific equipments
Stereo microscope MZ6 Leica

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Diesen Artikel zitieren
Roux-en-Y Gastric Bypass Procedure: A Bariatric Surgical Procedure for Gastric Resectioning in Murine Model. J. Vis. Exp. (Pending Publication), e20815, doi: (2023).

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