Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
Published: April 30, 2023
Abstract
Source: Wu, K. et al. Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice. J. Vis. Exp. (2021)
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Solution and Perfusion Apparatus Preparation Assemble a constant flow Langendorff apparatus (commercially available or self-made). Figure 1 provides a simple schematic. Prepare the solutions according to the reagents given in Table 1 and Table …
Representative Results
Figure 1. Schematic diagram of an assembled modified Langendorff system. This system is economical and portable for the users. It has two parts of plastic tubing-one for the water (inner diameter, 8 mm) and one for the perfusate (inner diameter, 1.5 mm)-of which the distal end is connected to a PE medical three-way stopcock with a Luer lock. A glass bottle containing water an…
Offenlegungen
The authors have nothing to disclose.
Materials
| 2,3-butanedione monoxime (BDM) | Sigma-Aldrich | 31550 | |
| CaCl2 | Sigma-Aldrich | C4901 | |
| Glucose | Sigma-Aldrich | G7528 | |
| HEPES | Sigma-Aldrich | H3375 | |
| KCl | Sigma-Aldrich | P9333 | |
| KH2PO4 | Sigma-Aldrich | P5655 | |
| KHCO3 | Sigma-Aldrich | 237205 | |
| MgCl2 | Sigma-Aldrich | M8266 | |
| MgSO4 | Sigma-Aldrich | M7506 | |
| Na2HPO4 | Sigma-Aldrich | S7907 | |
| NaCl | Sigma-Aldrich | S7653 | |
| NaHCO3 | Sigma-Aldrich | S5761 | |
| Peristaltic pump | Longerpump | BT100-2J | |
| Taurine | Sigma-Aldrich | T0625 | |
| Water bath | JULABO | ED (v.2) |
Tags
Diesen Artikel zitieren
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart. J. Vis. Exp. (Pending Publication), e20749, doi: (2023).